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爱必信(上海)生物科技有限公司
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文献和实验which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments
MAbs (mAbs) are unique and versatile molecules that have found applications in research, in vitro and in vivo diagnostics, and the treatment of several diseases. Antibody technology has been revolutionized by the hybridoma technology for rnAb
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