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- 文献和实验
- 技术资料
- 英文名：
Isomaltodecaose DP10 linked to biotin and fluorescein (Linker-BT-FLUO A)
- 库存：
100+
- 供应商：
上海维亦特生物科技有限公司
分子量:2317.44
性状：Orange powder
储存：室温
溶解性：in water, PBS or HEPES
来源：Glycan moiety produced by extraction of dextran
附件：MS and 1H-NMR
糖链结构：Glcα1-6[Glcα1-6]8Glc-O-(CH2)3-NHCO-CH(NHCO-Biotin)-(CH2)4-NH-CS-NH-FITC
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文献和实验分子量:2317.44
性状：Orange powder
储存：室温
溶解性：in water, PBS or HEPES
来源：Glycan moiety produced by extraction of dextran
附件：MS and 1H-NMR
糖链结构：Glcα1-6[Glcα1-6]8Glc-O-(CH2)3-NHCO-CH(NHCO-Biotin)-(CH2)4-NH-CS-NH-FITC
, most commonly digoxygenin, biotin, and fluorescein. This approach involves a longer detection protocol than a direct enzyme labeling methodology (see Chapter 10 ) because of the additional steps involved in membrane blocking, antibody binding, and subsequent
. The intensity of the biotin-linked fluorescence is amplified by adding a layer of biotinylated goat anti-avidin antibody followed by another layer of fluorescein-avidin DCS. After washing off the excess stain, a fluorescence anti fade solution, e.g. p
Sequential RNA and DNA fluorescence in situ hybridization
) and air dry; Denature a biotin-labeled RNA FISH probe for 10 minutes at 85 °C and keep at 37 °C for 30 minutes (note 4); Add the RNA FISH probe as a drop onto a microscope slide. Place the coverslip cell-side down on top of the RNA FISH probe
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