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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
冷冻-20℃
- 保质期:
咨询
- 英文名:
5-(2,3,5-Tri-O-benzyl-beta-D-ribofuranosyl)-3-methyl-2-benzyloxypyridine
- 库存:
100+
- 供应商:
上海维亦特生物科技有限公司
- CAS号:
1846584-69-7
分子量:601.73
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文献和实验Alternatives to demethylate DNA in vivo other than 5-aza
) for it to work. Zebularine (1-(beta-D-ribofuranosyl)-1,2-dihydropyrimidin-2-one) is another nucleotide analogue that has been shown to last longer than 5-aza-dC (see Cheng et al 2004 Mol Biol Cell) and is not affected by deaminase activity. Zebularine
References 1. R. M. O’Connell, K. D. Taganov, M. P. Boldin, G. Cheng, D. Baltimore, MicroRNA-155 is induced during the macrophage inflammatory response. Proc. Natl. Acad. Sci. U.S.A. 104, 1604–1609 (2007).[Abstract/Free Full Text] 2. D
Sucrose Density Gradient Fractionation
Grow a 2 ml culture,24 hr.at 30℃ in selective mediaWhen culture is ready,use it to inoculate about 55 ml (50 ml plus 5 for O.D.600 readings)of selective media.Grow to mid-logarithmic phase (O.D.600 is about 1).This usually takes 10 hours (for 1ml
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