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- 文献和实验
- 技术资料
- 保存条件:
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- 英文名:
2-[(Dicyclopropylmethyl)amino]ethanol
- 库存:
100+
- 供应商:
上海维亦特生物
- CAS号:
1099052-30-8
2-[(Dicyclopropylmethyl)amino]ethanol
1099052-30-8
C9H17NO
155.24
No Data Available
No Data Available
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文献和实验2-[(Dicyclopropylmethyl)amino]ethanol
1099052-30-8
C9H17NO
155.24
No Data Available
No Data Available
analysis for relative quantitation of protein expression in microglial cells to elucidate the molecular mechanisms underlying microglial activation after ethanol exposure. The approach involves stable isotope labeling with amino acids in cell culture
Preparation of probes for in situ hybridization
nucleotides, amino-allyl nucleotides are incorporated almost as well as unmodified UTP. Transcription reactions are then phenol extracted, RNA is ethanol precipitated, resuspended in 1 x SSC, and unincorporated nucleotides are removed by two rounds of gel
Preparation of probes for in situ hybridization
oligonucleotides are the preferable probes at present. They are synthesized chemically, which allows for the incorporation of amino-modified nucleotides (amino-allyl T) at defined positions in the sequence. These free amines are chemically coupled to fluorophores
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