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(3Beta,20S)-21-O-[[Tris(isopropyll)silyl]-20-methyl-pregn-5-ene-3,21-diol
N/A
C₃₁H₅₆O₂Si
488.86
White Solid
Dichloromethane, Ethyl Acetate, Methanol
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文献和实验(3Beta,20S)-21-O-[[Tris(isopropyll)silyl]-20-methyl-pregn-5-ene-3,21-diol
N/A
C₃₁H₅₆O₂Si
488.86
White Solid
Dichloromethane, Ethyl Acetate, Methanol
200ml PEG6000 40% 37.21 4.0g 20g 40g 60g 80g H2 O mQ 62.79 6.75g 33.75g 67.5g 101.25g 135.0g p=1.075. PMSF (store at -20o C) Conc. Stock 20ml C7 H7 FO2 S 100mM 174.2g/M
Site-directed hydroxy radical mapping of nucleosome positions in vitro
12). Mapping Reaction A typical sample for mapping has a total volume of 10-20µl and contains 0.2-0.5µM reconstituted nucleosome, 50mM Tris-Cl pH 7.5 and appropriate monovalent salt. After desired experimental manipulation, place sample
Buffer, # N808-0189, and Ampli-Taq DNA polymerase, # N808-4015 store frozen, -20°C (Perkin Elmer, Norwalk, CT) Carbenicillin (Gibco-BRL, Rockville, MD) Ethanol (200 Proof USP Ethyl Alcohol) 1M Tris-HCl (pH 8) 0.5M NaEDTA (pH 8) T Low E Buffer
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