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- CAS号：
60644-20-4
Fructosyl Glycine a/b Mixture (Mixture of Diastereomers)
60644-20-4
C₈H₁₅NO₇
237.21
Light Beige to Dark Brown Solid
Water (Slightly)
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文献和实验Fructosyl Glycine a/b Mixture (Mixture of Diastereomers)
60644-20-4
C₈H₁₅NO₇
237.21
Light Beige to Dark Brown Solid
Water (Slightly)
Radioligand Binding Assays for the Glycine Site on N‐Methyl‐D‐Aspartate Receptors
. Ivanovic, A., Reilander, H., Laube, B. and Kuhse, J. 1998. Expression and initial characterization of a soluble glycine binding domain of the N‐methyl‐D‐aspartate receptor NR1 subunit. J. Biol. Chem. 273:19933
Procedure for Transfection of Mammalian Cells
in a CO2 incubator until the cells are 70-80% confluent. This will usually take 18-24 h. Prepare the following solutions in 12 x 75 mm sterile tubes: Solution A: For each transfection, dilute 2 μg DNA (plasmid) in 375 μl serum-free IMDM
-based Analysis)Proteinase K (Sigma)Proteinase K buffer (0.05 M tris-HCL, 0.001 M EDTA, 1% Tween 20, 0.1 mg/ml proteinase K, pH 8.0)Ampli Taq Gold Buffer (Perkin Elmer)dNTP mixture (Perkin Elmer)PrimersDEPC-treated H2OAmpli Taq Gold Polymerase (Perkin
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