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Mixture of 10,11-Dibromo-10,11-dihydro-5H-dibenz[b,f]azepine-5-carbonyl chloride & 10,11-Dibromo-10,11-dihydro-5H-dibenz[b,f]azepine-5-carbonyl Bromide
N/A
C₁₅H₁₀Br₂ClNO • C₁₅H₁₀Br₃NO
N/A
White to Off-White Solid
Chloroform (Slightly, Heated, Sonicated), DMSO (Slightly, Sonicated)
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文献和实验Mixture of 10,11-Dibromo-10,11-dihydro-5H-dibenz[b,f]azepine-5-carbonyl chloride & 10,11-Dibromo-10,11-dihydro-5H-dibenz[b,f]azepine-5-carbonyl Bromide
N/A
C₁₅H₁₀Br₂ClNO • C₁₅H₁₀Br₃NO
N/A
White to Off-White Solid
Chloroform (Slightly, Heated, Sonicated), DMSO (Slightly, Sonicated)
Measurement of Chloride Movement in Neuronal Preparations
. Cala, P.M. 1990. Principles of cell volume regulation: Ion flux pathways and the roles of anions. In Chloride Channels and Carriers in Nerve, Muscle, and Glial Cells (F.J. Alvarez‐Leefmans and J.M. Russell eds
CALCIUM CHLORIDE TRANSFORMATION OF BACTERIA WITH PLASMIDS
it with a pipet tip, and incubate the mixture on ice for 20 minutes. Heat shock the mixture for 2 minutes at 42℃ (37℃ is also fine). Transfer the bacterial suspension to 10 ml of L Broth without antibiotic. Shake for 45 minutes in a 37℃ incubator. Centrifuge
Cesium Chloride Purification of T7
1 molar. Centrifuge the lysate at 10,000 rpm for 10 min, Discard the cellular debris, and add 10 grams polyethylene glycol (PEG) m.w. 8000 (10% w/v) to the supernatant. Gently stir the mixture until the PEG has totally dissolved. Keep lysate on ice
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