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- CAS号：
22529-61-9
3-O-Benzyl-1,2-O-isopropylidene-alpha-D-glucofuranose
22529-61-9
C₁₆H₂₂O₆
310.34
Clear Colourless to Yellow Thick Oil
Chloroform (Slightly), Methanol (Slightly)
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文献和实验3-O-Benzyl-1,2-O-isopropylidene-alpha-D-glucofuranose
22529-61-9
C₁₆H₂₂O₆
310.34
Clear Colourless to Yellow Thick Oil
Chloroform (Slightly), Methanol (Slightly)
Replication timing by density transfer
in Ycomp+[12 C]-glucose+ alpha factor. [I don't particularly try to resuspend the cells in exactly the same volume they were in originally, but rather, in a volume that's convenient.] 4. Transfer to 37o C, wait for culture to reach 37o C
Replication timing by comparative hybridization
the culture to the 37o bath. 5. Once the culture has reached 37o C (it takes 10-15 min), add Pronase (0.01-0.05 mg/ml final conc) to release the cells from the alpha factor block. [You can add dry Pronase. I usually dissolve the Pronase in 5 ml
References 1. R. M. O’Connell, K. D. Taganov, M. P. Boldin, G. Cheng, D. Baltimore, MicroRNA-155 is induced during the macrophage inflammatory response. Proc. Natl. Acad. Sci. U.S.A. 104, 1604–1609 (2007).[Abstract/Free Full Text] 2. D
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