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- Hello Bio
- 英国
- HB7033
- 2026年03月01日
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- 技术资料
- 保存条件：
<p>+4°C or -20°C long-term. Protect from light.</p>
- 英文名：
MightyMount<sup>TM</sup> Antifade Fluorescence Mounting Medium with Propidium Iodide (hardset)
- 供应商：
Hello Bio
- 规格：
10mL
Overview
MightyMountTM Antifade Fluorescence Mounting Medium with propidium iodide (hardset) is an ideal formulation for prevention of photobleaching of fluorescent proteins and dyes during fluorescent imaging. It is easy to use with an ideal refractive index and provides effective prevention of photobleaching. This formulation contains propidium iodide which is a widely used red-fluorescent intercalating agent that binds and labels nucleic acids.
Applications: IHC(IF), ICC, Cellular imaging, Super-resolution microscopy
Mounting: Aqueous (hardset) - cures in approximately 1 hour at room temperature
Antifade: Yes
Counterstain: Propidium Iodide
Refractive index: ≈1.45 (initial) which then increases to ≈1.518 once cured
Other Mounting Media Products
We supply a full range of mounting media for a range of experimental needs:
Hardset:
- HB6966 - MightyMountTM Antifade Fluorescence Mounting Medium (hardset)
- HB8459 - MightyMountTM Antifade Fluorescence Mounting Medium with DAPI (hardset)
- HB7508 - MightyMountTM Antifade Fluorescence Mounting Medium with Phalloidin-TRITC (hardset)
Aqueous:
- HB9854 - MightyMountTM Antifade Fluorescence Mounting Medium (aqueous)
- HB7618 - MightyMountTM Antifade Fluorescence Mounting Medium with DAPI (aqueous)
- HB8761 - MightyMountTM Antifade Fluorescence Mounting Medium with Propidium Iodide (aqueous)
- HB9417 - MightyMountTM Antifade Fluorescence Mounting Medium with Phalloidin-TRITC (aqueous)
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文献和实验Tunel Procedure in Bovine Embryos 牛胚胎TUNEL检测凋亡
around the sample, add 16 µl of mounting medium (w/ antifade) to the slide and cover the sample with coverslip. 14) Determine the number of fluorescent nuclei. Red fluorescence indicates nonapoptotic cells and green/yellow (PI) or (Hoescht
TUNEL PROCEDURE IN BOVINE EMBRYOS
and add propidium iodide (0.5 µg/ml) for 1 h at room temperature. 12) Wash the slides 4 times in PBS/PVP (2 minutes each). 13) Dry the area around the sample, add 16 µl of mounting medium (w/ antifade) to the slide and cover
TUNEL PROCEDURE IN BOVINE EMBRYOS
. Dilute 1 µl RNase A (stock) with 999 µl PBS/PVP and then dilute this solution 1:1 with PBS/PVP to obtain a working solution (Use fresh). Mounting medium and antifade: ProLong Antifade Kit (Molecular
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