DNA-Free One-Step RT-qPCR 2× S

DNA-Free One-Step RT-qPCR 2× SuperMix (Taqman) is used for the quantitative analysis of RNA molecules. By utilizing Taqman probes for quantification, it eliminates false positives caused by primer dimers and non-specific amplification products. This product contains MMLV RTase 2.0, RNase Inhibitor, hot-start Taq DNA polymerase, dNTPs, and an optimized reaction buffer at a 2× concentration. For RT-qPCR, simply add the RNA template, primers, and DEPC-treated water to adjust the 2× SuperMix solution to a 1× concentration for the reaction. RNA sample types include total RNA, mRNA, ribosomal RNA, tRNA, and others. One of the core components, MMLV RTase 2.0, has been genetically engineered to significantly improve its thermal stability, making it resistant to 55-60℃. This allows the reverse transcription reaction to be performed at 50-55℃, eliminating the inhibitory effects of RNA secondary structures on reverse transcription. The buffer is specifically optimized for RT-qPCR, ensuring high activity for both MMLV RTase and Taq DNA polymerase, and the Taqman probes release high signal, making it highly suitable for Taqman probe-based one-step RT-qPCR.

Cat. No.: FORMT-1 (for 1ml)

Cat. No.: FORMT-5 (for 5ml)

Cat. No.: FORMT-20 (for 20ml)

Cat. No.: FORMT-50 (for 50ml)

Description

DNA-Free One-Step RT-qPCR 2× SuperMix (Taqman) is used for the quantitative analysis of RNA molecules. By utilizing Taqman probes for quantification, it eliminates false positives caused by primer dimers and non-specific amplification products. This product contains MMLV RTase 2.0, RNase Inhibitor, hot-start Taq DNA polymerase, dNTPs, and an optimized reaction buffer at a 2× concentration. For RT-qPCR, simply add the RNA template, primers, and DEPC-treated water to adjust the 2× SuperMix solution to a 1× concentration for the reaction. RNA sample types include total RNA, mRNA, ribosomal RNA, tRNA, and others. One of the core components, MMLV RTase 2.0, has been genetically engineered to significantly improve its thermal stability, making it resistant to 55-60℃. This allows the reverse transcription reaction to be performed at 50-55℃, eliminating the inhibitory effects of RNA secondary structures on reverse transcription. The buffer is specifically optimized for RT-qPCR, ensuring high activity for both MMLV RTase and Taq DNA polymerase, and the Taqman probes release high signal, making it highly suitable for Taqman probe-based one-step RT-qPCR.

Features

• Completely free from bacterial DNA.
• One-step RT-qPCR reduces operation time.
• Prevents contamination from multiple steps.
• Quantification of RNA fragments (≤3 kb).
• Hot-start Taq enzyme reduces false positive signals due to non-specific amplification, offering high amplification efficiency and good stability.
• Taqman probes eliminate false positives caused by primer dimers and non-specific amplification products.
• MMLV RTase 2.0 allows reverse transcription at 50-55℃, eliminating the inhibitory effects of RNA secondary structures.

Applications

• Quantitative RT-qPCR of RNA samples (mRNA, rRNA, etc.), using Taqman probe-based quantification.