DNA-Free One-Step RT-qPCR 2× S

DNA-Free One-Step RT-qPCR 2× SuperMix (SYBR Green) uses SYBR Green I intercalating dye for the quantitative analysis of RNA molecules. This product contains MMLV RTase 2.0, RNase Inhibitor, hot-start Taq DNA polymerase, dNTPs, SYBR Green, and an optimized reaction buffer at a 2× concentration. For RT-qPCR, simply add the template, primers, and DEPC-treated water to adjust the 2× SuperMix solution to a 1× concentration for the reaction. RNA sample types include total RNA, mRNA, ribosomal RNA, tRNA, and others. One of the core components, MMLV RTase 2.0, has been genetically engineered to significantly improve its thermal stability, making it resistant to 55-60℃. This allows the reverse transcription reaction to be performed at 50-55℃, eliminating the inhibitory effects of RNA secondary structures on reverse transcription. The buffer is specifically optimized for RT-qPCR, ensuring high activity for both MMLV RTase and Taq DNA polymerase, making it highly suitable for one-step RT-qPCR. Some qPCR instrument models may require ROX reference dye.

Cat. No.: FORMG-1 (for 1ml)

Cat. No.: FORMG-5 (for 5ml)

Cat. No.: FORMG-20 (for 20ml)

Cat. No.: FORMG-50 (for 50ml)

Description

DNA-Free One-Step RT-qPCR 2× SuperMix (SYBR Green) uses SYBR Green I intercalating dye for the quantitative analysis of RNA molecules. This product contains MMLV RTase 2.0, RNase Inhibitor, hot-start Taq DNA polymerase, dNTPs, SYBR Green, and an optimized reaction buffer at a 2× concentration. For RT-qPCR, simply add the template, primers, and DEPC-treated water to adjust the 2× SuperMix solution to a 1× concentration for the reaction. RNA sample types include total RNA, mRNA, ribosomal RNA, tRNA, and others. One of the core components, MMLV RTase 2.0, has been genetically engineered to significantly improve its thermal stability, making it resistant to 55-60℃. This allows the reverse transcription reaction to be performed at 50-55℃, eliminating the inhibitory effects of RNA secondary structures on reverse transcription. The buffer is specifically optimized for RT-qPCR, ensuring high activity for both MMLV RTase and Taq DNA polymerase, making it highly suitable for one-step RT-qPCR. Some qPCR instrument models may require ROX reference dye.

Features

• Completely free from bacterial DNA.
• One-step RT-qPCR reduces operation time.
• Prevents contamination from multiple steps.
• Quantification of RNA fragments (≤3 kb).
• Hot-start Taq enzyme reduces false positive signals due to non-specific amplification, offering high amplification efficiency and good stability.
• MMLV RTase 2.0 allows reverse transcription at 50-55℃, eliminating the inhibitory effects of RNA secondary structures.

Applications

• Quantitative RT-qPCR of RNA samples (mRNA, rRNA, etc.), using SYBR Green dye.