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- DFTAB5-500 (for 500U)
- 2026年01月09日
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DNA-Free Alkaline Phosphatase (TAB5) catalyzes the hydrolysis of phosphate monoesters but does not catalyze the hydrolysis of phosphate diesters or triesters. Alkaline Phosphatase (TAB5) is derived from psychrophilic bacteria, exhibiting high activity in the temperature range of 25-50℃. Due to its origin from psychrophilic bacteria, it has very low thermal stability and can be irreversibly inactivated by heat treatment at 65℃ for 15 minutes or 70℃ for 5 minutes.
Cat. No.: DFTAB5-500 (for 500U)
Cat. No.: DFTAB5-2500 (for 2500U)
Description
DNA-Free Alkaline Phosphatase (TAB5) catalyzes the hydrolysis of phosphate monoesters but does not catalyze the hydrolysis of phosphate diesters or triesters. Alkaline Phosphatase (TAB5) is derived from psychrophilic bacteria, exhibiting high activity in the temperature range of 25-50℃. Due to its origin from psychrophilic bacteria, it has very low thermal stability and can be irreversibly inactivated by heat treatment at 65℃ for 15 minutes or 70℃ for 5 minutes.
Our DNA-Free enzymes are all recombinant proteins and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.
Unit Definition
One unit is defined as the amount of enzyme required to dephosphorylate 1 μg of pUC19 DNA digested with HindIII (5' overhangs), EcoRV (blunt ends), or PstI (3' overhangs) within 30 minutes at 37℃. Dephosphorylation is confirmed by the inhibition of >95% DNA recircularization in a self-ligation reaction (assessed by transforming E. coli competent cells with the ligation products).
Features
- Completely free from bacterial DNA.
- High activity within the temperature range of 25-50℃.
- Highly thermolabile, completely inactivated by heating at 65℃ for 15 minutes or 70℃ for 5 minutes.
Applications
- Removal of 5’ phosphate groups from DNA molecules.
- Pre-treatment of PCR products for sequencing when combined with Exonuclease I.
Storage
Store at -20℃ for up to 2 years. Avoid repeated freeze-thaw cycles.
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文献和实验in solution c. for 5-15 minutes at room temperature. Wash, counterstain in haematoxylin, wash. Mount in phosphate buffered glycerine jelly RESULTS Alkaline phosphatase activity - Red Nuclei - Blue
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity 1. Obtain embryo samples, tube of AP substrate buffer and tube of phosphate buffered saline ( PBS ) for each group. Allow embryos
1.1 General information Endothelial cells possess an endogenous alkaline phosphatase (AP) activity. The enzymatic activity of AP is not restricted to the endothelial lineage. In medaka embryos, somite cells during early developmental stages
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