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- SBS
- DFBLF-200 (for 200U)-1
- 2026年01月07日
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DNA-Free Bst DNA polymerase (Large Fragment), also known as Bst DNA polymerase large fragment, is a proteolytic fragment of DNA polymerase derived from the thermophilic bacterium Bacillus stearothermophilus. It possesses 5´→3´ DNA polymerase activity but lacks 5´→3´ exonuclease activity.
Cat. No.: DFBLF-200 (for 200U)
Cat. No.: DFBLF-1k (for 1000U)
Description
DNA-Free Bst DNA polymerase (Large Fragment), also known as Bst DNA polymerase large fragment, is a proteolytic fragment of DNA polymerase derived from the thermophilic bacterium Bacillus stearothermophilus. It possesses 5´→3´ DNA polymerase activity but lacks 5´→3´ exonuclease activity.
Our DNA-Free Bst DNA polymerase (Large Fragment) is a recombinant protein and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.
Unit Definition
One unit is defined as the amount of enzyme required to incorporate 10 nmol dNTP into acid-insoluble material within 30 minutes at 65℃ under 1× Buffer reaction conditions.
Features
- Completely free from bacterial DNA.
- Lacks 5´→3´ exonuclease activity.
- Extremely strong strand displacement synthesis ability.
- Heat inactivation at 85℃ for 5 minutes.
Applications
- DNA isothermal amplification.
- Amplification of GC-rich DNA.
- Rapid amplification of nanogram-scale DNA templates.
- Suitable for high-temperature strand displacement DNA synthesis.
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文献和实验), where we have overcome both the size limitations and the restrictions of the cloning/subcloning procedures used in the Red/ET recombineering system. We describe the transfer of a very large DNA fragment (~55 kb) from one BAC into another unrelated BAC by the means
Radiolabeling of DNA with the Klenow Fragment of DNA Polymerase
The Klenow fragment of DNA polymerase I is a proteolytic fragment obtained by the treatment of DNA polymerase I with subtilisin. The fragment still has the polymerase and 3′–5′ exonuclease activity, but lacks the 5′–3′ exonuclease activity
Bst-catalyzed radiolabeled DNA sequencing
Bst DNA polymerase-catalyzed radiolabeled two-step sequencing reactions are modified from those presented earlier by altering the absolute amounts and the relative deoxy/dideoxynucleotide ratios in the termination mixes
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