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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
见斯达特(Starter)网站
- 英文名:
Recombinant Tobacco Etch Virus Protease (rTEV)
- 库存:
999
- 供应商:
斯达特生物
- 规格:
1kU
产品货号: UA070003
产品名称: Recombinant Tobacco Etch Virus Protease (rTEV)
中文名称: 重组烟草蚀纹病毒蛋白酶 (rTEV)
产品分类: 融合标记切割酶
宿主来源: Tobacco Etch Virus
抗原名称/别名: rTEV,TEV,RTEV
Accession:
方法学应用: 见斯达特生物网站
反应种属: 见斯达特生物网站
标记: His Tag
缓冲体系:
20mM Tris-HCl, pH8.0, 150mM NaCl, 1mM EDTA, 5mM DTT, 50%(v/v) Glycerol
表达系统: E.coli
储存条件:
· 12 months from date of receipt, -20 to -70 °C as supplied.
· 6 months, -20 to -70 °C under sterile conditions after reconstitution.
· 1 week, 2 to 8 °C under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
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文献和实验Application of TEV Protease in Protein Production
method for the removal of affinity tags from recombinant fusions using a highly purified proteinase with an unparalleled degree of specificity. This proteinase, from the genome of tobacco etch virus (TEV), demonstrates specific proteolytic activity
Strategies to Optimize Protein Expression in E. coli
., Anderson, D.E., Cherry, S., Copeland, T.D., and Waugh, D.S. 2001. Tobacco etch virus protease: Mechanism of autolysis and rational design of stable mutants with wild‐type catalytic proficiency. Protein Eng. 14:993‐1000
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