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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白的浓度
- 适应物种:
大鼠
- 标记物:
Cyp2b12/ Rat Cyp2b12
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.78pg/ml
- 规格:
96tests
Rat Cyp2b12 ELISA试剂盒
Product Name:Rat Cyp2b12 ELISA试剂盒
Packing:96T
Catalog No.:Rat Cyp2b12 ELISA试剂盒
Gene Name:Rat Cyp2b12
Detect Range:78-5000pg/mL
Sensitivity:46.79pg/mL
Target Protein Name:Rat Cyp2b12
Alternative Name:Rat Cyp2b12
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Rat Cyp2b12 ELISA KIT allows for the in vitro quantitative determination of Rat Cyp2b12 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Rat Cyp2b12 ELISA KIT has been pre-coated with an Rat Cyp2b12 antibody specific to Rat Cyp2b12 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rat Cyp2b12 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat Cyp2b12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rat Cyp2b12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
人CYP2D6 酶联免疫分析 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中 CYP2D6 的含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中人 CYP2D6 水平。用纯化的人 CYP2D6 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 CYP2D6 ,再与 HRP 标记的 CYP2D6 抗体结合,形成抗体 - 抗原 - 酶标抗体复合物,经过彻底洗涤
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