Mouse Alpha-amylase 1, Amy1 EL

Mouse Alpha-amylase 1, Amy1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY!

Aletnative

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Intended use

Mouse AMY1 ELISA KIT allows for the in vitro quantitative determination of mouse alpha-amylase 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Mouse AMY1 ELISA KIT has been pre-coated with an antibody specific to Alpha-amylase 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Alpha-amylase 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Alpha-amylase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Alpha-amylase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 78-5000 pg/ml

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