Mouse Aconitate hydratase, mit

Mouse Aconitate hydratase mitochondrial, ACO2 ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Mouse ACO2 ELISA KIT ,Mouse ACONM ELISA KIT ,Mouse HEL-S-284 ELISA KIT ,Mouse ICRD ELISA KIT ,Mouse OCA8 ELISA KIT ,Mouse OPA9 ELISA KIT ,Mouse aconitase 2 ELISA KIT ,Mouse  mitochondrial ELISA KIT ,Mouse citrate hydro-lyase ELISA KIT ,Mouse epididymis secretory sperm binding protein Li 284 ELISA KIT ,Mouse mitochondrial aconitase ELISA KIT ,Mouse aconitase 2 ELISA KIT

Intended use

Mouse ACO2 ELISA kit allows for the in vitro quantitative determination of Mouse ACO2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Mouse ACO2 ELISA kit has been pre-coated with an antibody specific to ACO2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ACO2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ACO2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ACO2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.31-20ng/ml

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