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- 详细信息
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- 文献和实验
- 技术资料
- 英文名：
HT-22 Mouse Hippocampal Neuronal Cell Line
- 规格：
T25/冻存管
细胞名称：HT-22 Mouse Hippocampal Neuronal Cell Line
货号：SCC129
种属：mouse
细胞系描述：神经谱系细胞
应用：
EMD Millipore拥有仅用于研究用途而非任何商业用途销售本产品的有限许可证。排除的商业用途包括但不限于制造，提供服务、治疗、诊断和预防用途以及任何其他商业用途。未经授权和禁止买方将本产品用于研究以外的任何目的。
研究类别
神经科学
毒性
质量：
• 每瓶含有≥ 1X10^6 个活细胞。
•Charles River动物诊断服务通过小鼠Essential CLEAR小组对细胞进行传染病检测，结果为阴性。
•通过Charles River动物诊断服务的污染透明小组评估，证实细胞为小鼠来源，对大鼠、中国仓鼠、金色黄叙利亚仓鼠、人和非人灵长类动物（NHP）的种间污染物呈阴性。
•细胞对支原体污染呈阴性。
储存及稳定性：储存在液氮中。在初始解冻后，细胞可以培养至少10代，而不会显著影响细胞标志物的表达和功能。
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文献和实验Cui Y, Xiong Y, Li H, Zeng M, Wang Y, Li Y, Zou X, Lv W, Gao J, Cao R, Meng L, Long J, Liu J, Feng Z. Chalcone-Derived Nrf2 Activator Protects Cognitive Function via Maintaining Neuronal Redox Status. Antioxidants (Basel). 2021 Nov 15;10(11):1811. doi: 10.3390/antiox10111811. PMID: 34829682; PMCID: PMC8615013.
Lu Y, Wang J, Tang F, Pratap UP, Sareddy GR, Dhandapani KM, Capuano A, Arvanitakis Z, Vadlamudi RK, Brann DW. Regulation and Role of Neuron-Derived Hemoglobin in the Mouse Hippocampus. Int J Mol Sci. 2022 May 11;23(10):5360. doi: 10.3390/ijms23105360. PMID: 35628182; PMCID: PMC9140924.
Eom SH, Hong GL, Kang HB, Lee NS, Kim DK, Jeong YG, Kim CS, Yoo YC, Lee BH, Jung JY, Kim DS, Han SY. Neuroprotective Effects of Water Extract from Brown Algae Petalonia binghamiae in an Experimental Model of Focal Cerebral Ischemia In Vitro and In Vivo. Curr Issues Mol Biol. 2023 Oct 17;45(10):8427-8443. doi: 10.3390/cimb45100531. PMID: 37886974; PMCID: PMC10605114.
Jing W, Zhang T, Liu J, Huang X, Yu Q, Yu H, Zhang Q, Li H, Deng M, Zhu LQ, Du H, Lu Y. A circuit of COCH neurons encodes social-stress-induced anxiety via MTF1 activation of Cacna1h. Cell Rep. 2021 Dec 28;37(13):110177. doi: 10.1016/j.celrep.2021.110177. PMID: 34965426.
. Here, we describe a useful “sandwich” co-culture system where postnatal mouse hippocampal neurons are grown suspended above a feeder layer of mouse hippocampal astrocytes. We demonstrate that neurons in these cultures remain healthy beyond 3 weeks
Banker Culture Hippocampal Dissection
After 3-4 hours the coverslips should be flipped cell-side down onto the dish of glia. *Before starting the dissociation , make sure the 100mm dishes containing coverslips and neuronal medium, plus the glial dishes with neuronal
Measurement of Chloride Movement in Neuronal Preparations
of a maximally effective concentration of GABA (100 µM) to the perfusion medium. At 20 min after application, GABA produced an average Δ F of 22%. Note that one cell was relatively insensitive to GABA. View
