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- 技术资料
- 英文名：
HSC-T6 Rat Hepatic Stellate Cell Line
- 规格：
T25/冻存管
细胞名称：HSC-T6 Rat Hepatic Stellate Cell Line
货号：SCC069
种属：rat
细胞系描述：Hepatocyte Cells
应用：
Research Category
Metabolism
Infectious Diseases
Inflammation & Immunology
Stem Cell Research
Research Sub Category
Liver Physiology
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation.
组分：1) ≥1X10^6 viable HSC-T6 Rat Hepatic Stellate Cells: (Catalog No. SCC069). Store in liquid nitrogen.
质量：
• Each vial contains ≥ 1X10^6 viable cells.
• Cells tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
• Cells tested negative for mycoplasma contamination.
储存及稳定性：HSC-T6 Rat Hepatic Stellate Cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
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文献和实验Wu JS, Chiu V, Lan CC, Wang MC, Tzeng IS, Kuo CY, Hsieh PC. Chrysophanol Prevents Lipopolysaccharide-Induced Hepatic Stellate Cell Activation by Upregulating Apoptosis, Oxidative Stress, and the Unfolded Protein Response. Evid Based Complement Alternat Med. 2020 Jul 4;2020:8426051. doi: 10.1155/2020/8426051. PMID: 32714424; PMCID: PMC7355365.
Liang H, Huang K, Su T, Li Z, Hu S, Dinh PU, Wrona EA, Shao C, Qiao L, Vandergriff AC, Hensley MT, Cores J, Allen T, Zhang H, Zeng Q, Xing J, Freytes DO, Shen D, Yu Z, Cheng K. Mesenchymal Stem Cell/Red Blood Cell-Inspired Nanoparticle Therapy in Mice with Carbon Tetrachloride-Induced Acute Liver Failure. ACS Nano. 2018 Jul 24;12(7):6536-6544. doi: 10.1021/acsnano.8b00553. Epub 2018 Jun 26. PMID: 29943967; PMCID: PMC6373867.
Nanda I, Steinlein C, Haaf T, Buhl EM, Grimm DG, Friedman SL, Meurer SK, Schröder SK, Weiskirchen R. Genetic Characterization of Rat Hepatic Stellate Cell Line HSC-T6 for In Vitro Cell Line Authentication. Cells. 2022 May 29;11(11):1783. doi: 10.3390/cells11111783. PMID: 35681478; PMCID: PMC9179542.
Friedman SL, Weiskirchen R. Working with Immortalized Hepatic Stellate Cell Lines. Methods Mol Biol. 2023;2669:129-162. doi: 10.1007/978-1-0716-3207-9_8. PMID: 37247058; PMCID: PMC12168884.
Isolation and Culture of Hepatic Stellate Cells
Hepatic stellate cells (HSCs) are routinely prepared by collagenase/pronase digestion of liver using a perfusion system and subsequent fractionation of the heterogenous cell suspension on continuous density gradients made out of Nycodenz
【摘要】 目的：探讨高迁移率族蛋白B1（HMGB1）对肝星状细胞(hepatic stellate cell HSC)合成细胞外基质(extracellular matrix ECM)的影响。 方法：剂量效应组分别用含HMGB1 0 mg/L,0.1 mg/L,1.0 mg/L,10 mg/L，20 mg/L和40 mg/L的HMGB1培养液培养HSC-T6 6 h；时间效应组以含有10 mg/L HMGB1的培养液培养0 h、6 h、12 h、18 h和24 h,对照组以不含HMGB
Derivation, Maintenance, and Characterization of Rat Embryonic Stem Cells In Vitro
describes the long-term cultivation of an alkaline phosphatase-positive rat embryonic stem cell-like line (RESC) and their differentiation into neuronal, endothelial, and hepatic lineages. The RESCs can be characterized by typical growth in single cells
