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- 文献和实验
- 技术资料
- 英文名：
Human Cardiomyocyte Cell Line
- 规格：
T25/冻存管
细胞名称：AC16 Human Cardiomyocyte Cell Line
货号：SCC109
种属：human
细胞系描述：心肌细胞
应用：
研究类别
心脏
心血管疾病
毒性
该产品可出售给学术机构或非营利性实体以供研究使用。
质量：
• 每小瓶含有≥ 1X10^6个活细胞。
•通过PCR检测细胞，对HPV-16、HPV-18、甲型、乙型、丙型肝炎和HIV-1 & 2型病毒呈阴性。
•细胞对支原体污染呈阴性。
•通过STR分析对每批细胞进行基因分型，以验证细胞系的唯一身份。
储存及稳定性：AC16人心肌细胞株应保存在液氮中。在初始解冻后，细胞可以培养至少10代，而不会显著影响细胞标志物的表达和功能。
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文献和实验Bouin A, Vu MN, Al-Hakeem A, Tran GP, Nguyen JHC, Semler BL. Enterovirus-Cardiomyocyte Interactions: Impact of Terminally Deleted Genomic RNAs on Viral and Host Functions. J Virol. 2023 Jan 31;97(1):e0142622. doi: 10.1128/jvi.01426-22. Epub 2022 Dec 8. PMID: 36475766; PMCID: PMC9888282.
Wu X, Zhu J, Wei Y, Guan X, Zhang Y, Chen W, Gao B. MicroRNA-663 participates in myocardial fibrosis through interaction with TGF-β1. Exp Ther Med. 2019 Oct;18(4):3172-3176. doi: 10.3892/etm.2019.7902. Epub 2019 Aug 16. PMID: 31572558; PMCID: PMC6755453.
Isolation and Differentiation of Human Cardiomyocyte Progenitor Cells into Cardiomyocytes
To date, there is no suitable in vitro model to study human adult cardiac cell biology. Here, we describe a method for efficient isolation and expansion of human cardiomyocyte progenitor cells (CMPCs) from cardiac surgical waste
Online Verification of Human Cell Line Identity by STR DNA Typing
The main prerequisition for any research, development, or production programs involving cell lines is whether a cell line is authentic or not. Microsatellites in the human genome harboring short tandem repeat (STR) DNA markers allow
The method described here to differentiate mouse embryonic stem (ES) cells into cardiomyocytes is adapted from Maltsev et al. and results in a high percentage of spontaneously beating cardiomyocyte-like cells. In order
