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- 技术资料
- 英文名：
O9-1 Mouse Cranial Neural Crest Cell Line
- 规格：
T25/冻存管
细胞名称：O9-1 Mouse Cranial Neural Crest Cell Line
货号：SCC049
种属：mouse
细胞系描述：Neural Lineage Cells
应用：
Research Category
Neuroscience
Stem Cell Research
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation.
组分：1) ≥1X10^6 viable O9-1 Mouse Cranial Neural Crest Cell Line: (Catalog No. SCC049). Derived from mass cultures of Wnt1-Cre; R26R-GFP reporter-expressing cranial neuronal crest cells from E8.5 mouse embryos.
质量：
• Each vial contains ≥ 1X106 viable cells at passage 20 -22
• Cells tested negative for infectious disease by a murine PCR panel (Mouse Essential CLEAR Panel by Charles River Animal Diagnostic Services)
• Cells tested negative for mycoplasma contamination
储存及稳定性：O9-1 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality.
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文献和实验Kamalakar A, McKinney JM, Salinas Duron D, Amanso AM, Ballestas SA, Drissi H, Willett NJ, Bhattaram P, García AJ, Wood LB, Goudy SL. JAGGED1 stimulates cranial neural crest cell osteoblast commitment pathways and bone regeneration independent of canonical NOTCH signaling. Bone. 2021 Feb;143:115657. doi: 10.1016/j.bone.2020.115657. Epub 2020 Sep 25. PMID: 32980561; PMCID: PMC9035226.
Amanso AM, Kamalakar A, Bitarafan S, Abramowicz S, Drissi H, Barnett JV, Wood LB, Goudy SL. Osteoinductive effect of soluble transforming growth factor beta receptor 3 on human osteoblast lineage. J Cell Biochem. 2021 May;122(5):538-548. doi: 10.1002/jcb.29888. Epub 2021 Jan 22. PMID: 33480071.
Inubushi T, Nakanishi Y, Abe M, Takahata Y, Nishimura R, Kurosaka H, Irie F, Yamashiro T, Yamaguchi Y. The cell surface hyaluronidase TMEM2 plays an essential role in mouse neural crest cell development and survival. PLoS Genet. 2022 Jul 15;18(7):e1009765. doi: 10.1371/journal.pgen.1009765. PMID: 35839257; PMCID: PMC9328550.
In Vitro Analysis of Mouse Mesencephalic Neural Crest Development
Figure 3.23.1 Schematic diagram of mouse mesencephalic neural crest cell cultures. The lines numbered 1 to 3 in A show dissection procedures for the isolation of the mesencephalic neural fold: Separate off the cranial region of the forebrain
Interspecific Chimeras: Transplantation of Neural Crest Between Mouse and Chick Embryos
for study. Two other labs have studied mouse neural crest or neural tube grafts in chick embryos (3 ,4 ). Our experience has been with grafting and tracking the cardiac neural crest. This population of cells presents some unique problems because the hosts
transgene with a focus on tracking cardiac neural crest cells using the Wnt1-Cre and R26R mouse lines. We also provide a protocol using fluorescence-activated cell sorting for collecting neural crest cells for further analysis. These protocols can be used
