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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSMBIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
猪
- 标记物:
Porcine Pepsin A,PGA
- 样本:
猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Porcine Pepsin A, PGA ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Pepsin A, PGA5,pepsin A-5; pepsin A; PGA3; PGA4; pepsinogen A5; pepsinogen-5; pepsinogen 5, group I (pepsinogen A)
Search name
PORCINE Pepsin A ELISA Kit, PORCINE Pepsin ELISA Kit,PORCINE PGA5 ELISA KIT,PORCINE pepsin A-5 ELISA KIT,PORCINE PGA3 ELISA KIT, PORCINE pepsinogen A5 ELISA KIT, PORCINE pepsinogen-5 ELISA KIT, PORCINE pepsinogen 5 ELISA KIT, Pepsin A ELISA Kit, Pepsin ELISA Kit, PGA5 ELISA KIT,pepsin A-5 ELISA KIT, PGA3 ELISA KIT,pepsinogen A5 ELISA KIT,pepsinogen-5 ELISA KIT,pepsinogen 5 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Porcine pepsin a,Pepsin A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pepsin A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pepsin A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pepsin A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pepsin A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Porcine-Pepsin A-PGA-ELISA-Kit
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文献和实验to crystallize proteins has obviously improved, and this web page highlights a few of the methods currently available. Figure 2. Three-dimensional structure of porcine pepsin. Pepsin was the first globular protein crystal used successfully in x-ray
: PI和Hoechst33342都是sigma公司的粉剂,不是kit,2、具体方法: 将PI和Hoechst33342的终浓度调到10μg/mg就行,用PBS,Hank's液、生理盐水或D液任何一种溶解都可以。Hoechst33342染色37℃10min就达到饱和,PI在4℃10~20min就行。3、文献出处: 《细胞实验指南》上册,p102-125,现代生物技术译从--科学出版社, 有好几篇文献,只列出其中的一个: Ha HC, Snyder SH. Poly(ADP-ribose
进口 F6436 Porcine Insulin ELISA* ELISA 96T 3500 原装进口 F6437 Rat High Range Insulin* ELISA 96T 3500
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