Rabbit Interleukin-1 beta,IL1B

Rabbit Interleukin- 1 beta, IL- 1b ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Interleukin-1 beta(IL-1 beta),;Catabolin,; interleukin-1 beta; IL-1beta; IL1beta; IL-1 beta; interleukin 1, beta

Search name
Rabbit Interleukin-1 beta ELISA Kit, Rabbit IL-1b ELISA Kit, Rabbit Interleukin-1b ELISA Kit, Rabbit IL-1beta ELISA Kit, Rabbit
Catabolin ELISA KIT,Rabbit IL1F2 ELISA KIT, Interleukin-1 beta ELISA Kit, IL-1b ELISA Kit, Interleukin-1b ELISA Kit, IL-1beta
ELISA Kit, Catabolin ELISA KIT, IL1F2 ELISA KIT

Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rabbit interleukin-1 beta,IL-1 beta concentrations in serum,
Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-1 beta. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-1 beta and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain IL-1 beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-1 beta in the samples is then determined by comparing
the O.D. of the samples to the standard curve.