- ￥3480
- LSMBIO
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0572Rb
- 2025年07月22日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
兔
- 标记物：
Rabbit Collagen Type II
- 样本：
兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rabbit COLLAGEN TYPE II ELISA KIT
Packing 96Tests
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Gene Name Col II/ COL2A1
Protein Name Collagen Type II
Alternative Name
Collagen Type II,type II collagen,collagen type II alpha 1 chain; ANFH; AOM; COL11A3; SEDC; STL1; collagen alpha-1(II) chain; alpha-1 type II collagen; arthroophthalmopathy, progressive (Stickler syndrome); cartilage collagen; chondrocalcin; collagen II, alpha-1 polypeptide; collagen, type II, alpha 1
Intended use
Rabbit COLLAGEN TYPE II ELISA KIT allows for the in vitro quantitative determination of COLLAGEN TYPE II concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
| Reagent | Quantity |
| Assay plate | 1 |
| Standard | 2 |
| Sample Diluent | 1 × 20mL |
| Assay Diluent A | 1 × 10mL |
| Assay Diluent B | 1 × 10mL |
| Detection Reagent A | 1 × 120μL |
| Detection Reagent B | 1 × 120μL |
| Wash Buffer(25 x concentrate) | 1 × 30mL |
| Substrate | 1 × 10mL |
| Stop Solution | 1 × 10mL |
| Plate sealer | 5 |
Test principle
The microtiter plate provided in Rabbit COLLAGEN TYPE II ELISA KIT has been pre-coated with an COLLAGEN TYPE II antibody specific to COLLAGEN TYPE II. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for COLLAGEN TYPE II and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain COLLAGEN TYPE II, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of COLLAGEN TYPE II in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storage
Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20
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兔II型胶原(Collagen II)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 兔II 型胶原 ( Collagen II )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗兔 Collagen II 单抗包被于酶标板上，标准品和样品中的 Collagen
猪II型胶原(Collagen II)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 猪II 型胶原 ( Collagen II )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗猪 Collagen II 单抗包被于酶标板上，标准品和样品中的 Collagen II与单抗
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