- ￥3480
- LSMBIO
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0525Rb
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
兔
- 标记物：
Rabbit Tissue-type Plasiminogen Actilyse
- 样本：
兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rabbit Tissue-type Plasiminogen Actilyse ELISA KIT
Packing 96Tests
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Gene Name T-PA
Protein Name Tissue-type Plasiminogen Actilyse
Alternative Name
Rabbit Tissue- type Plasiminogen Actilyse ELISA KIT ,Rabbit PLAT ELISA KIT ,Rabbit T-PA ELISA KIT ,Rabbit TPA ELISA KIT ,Rabbit tissue-type plasminogen activator ELISA KIT ,Rabbit alteplase ELISA KIT ,Rabbit plasminogen/activator kringle ELISA KIT ,Rabbit reteplase ELISA KIT ,Rabbit t-plasminogen activator ELISA KIT ,Rabbit plasminogen activator tissue type ELISA KIT
Intended use
Rabbit Tissue-type Plasiminogen Actilyse ELISA KIT allows for the in vitro quantitative determination of Tissue-type Plasiminogen Actilyse concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
| Reagent | Quantity |
| Assay plate | 1 |
| Standard | 2 |
| Sample Diluent | 1 × 20mL |
| Assay Diluent A | 1 × 10mL |
| Assay Diluent B | 1 × 10mL |
| Detection Reagent A | 1 × 120μL |
| Detection Reagent B | 1 × 120μL |
| Wash Buffer(25 x concentrate) | 1 × 30mL |
| Substrate | 1 × 10mL |
| Stop Solution | 1 × 10mL |
| Plate sealer | 5 |
Test principle
The microtiter plate provided in Rabbit Tissue-type Plasiminogen Actilyse ELISA KIT has been pre-coated with an Tissue-type Plasiminogen Actilyse antibody specific to Tissue-type Plasiminogen Actilyse. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Tissue-type Plasiminogen Actilyse and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tissue-type Plasiminogen Actilyse, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Tissue-type Plasiminogen Actilyse in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storage
Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20
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