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- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
兔
- 标记物:
Rabbit High mobility group protein B1,HMGB1
- 样本:
兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rabbit High mobility group protein B1, HMGB1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
High mobility group protein B1,;High mobility group protein 1(HMG-1),; HMGB1,high mobility group protein B1; HMG1; HMG3; SBP-1;
Amphoterin; HMG-1; Sulfoglucuronyl carbohydrate binding protein; high mobility group protein 1; high-mobility group (nonhistone
chromosomal) protein 1; high-mobility group box 1; high mobility group box 1
Search name
Rabbit High mobility group protein B1 ELISA Kit, Rabbit HMGB1 ELISA Kit, Rabbit High mobility group protein 1 ELISA Kit, Rabbit HMG-1 ELISA Kit, Rabbit HMG3 ELISA Kit, Rabbit SBP-1 ELISA Kit, Rabbit Amphoterin ELISA Kit, Rabbit HMG-1 ELISA Kit, Rabbit Sulfoglucuronyl carbohydrate binding protein ELISA Kit, Rabbit nonhistone chromosomal protein 1 ELISA Kit, Rabbit high-mobility group box 1 ELISA Kit, Rabbit high mobility group box 1 ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rabbit High mobility group protein B1 concentrations in serum,
Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to High mobility group protein B1. Standards or
samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for High
mobility group protein B1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then
a TMB substrate solution is added to each well. Only those wells that contain High mobility group protein B1, biotin-conjugated antibody
and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric
acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of High
mobility group protein B1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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