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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
兔
- 标记物:
Rabbit Matrix metalloproteinase 13,MMP13
- 样本:
兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rabbit Matrix metalloproteinase 13, MMP13 ELISA kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
MMP13,CLG3; MANDP1; MMP-13; collagenase 3; matrix metallopeptidase 13; matrix metalloproteinase 13; matrix metallopeptidase 13, Matrix metalloproteinase-13, UMRCASE,
Search name
Rabbit MMP13 ELISA KIT ,Rabbit CLG3 ELISA KIT ,Rabbit MANDP1 ELISA KIT ,Rabbit MMP-13 ELISA KIT ,Rabbit collagenase 3 ELISA KIT ,Rabbit matrix metallopeptidase 13 ELISA KIT ,Rabbit matrix metalloproteinase 13 ELISA KIT ,Rabbit matrix metallopeptidase 13 ELISA KIT ,Rabbit Matrix metalloproteinase-13 ELISA KIT ,Rabbit UMRCASE ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rabbit Collagenase 3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagenase 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Collagenase 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Collagenase 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Collagenase 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Matrix Metalloproteinase Expression in Lung Cancer
. Table 1 Matrix Metalloproteinase Classifi cation MMP family MMP number Common name
Assessing Matrix Metalloproteinase Expression and Activity in Hepatocellular Carcinomas
tissue inhibitors of matrix metalloproteinase (TIMPs). MMPs are involved in situations that require extracellular matrix remodeling, including wound healing, development, inflammation, fibrosis angiogenesis, and tumor invasion (2 –
Developmental and homeostatic remodeling of the extracellular matrix (ECM) is a highly regulated process orchestrated by a family of zinc-containing, calcium-dependent neutral proteases known as the matrix metallo-proteinases (MMP
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