Recombinant Zebrafish mcf2lb P
rotein (N-GST & C-His Tag), 重组斑马鱼mcf2lb蛋白 (N端GST标签 & C端His标签)- ￥1268
- abinScience
- France
- ZA481012
- 2026年03月03日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期：
1 year
- 英文名：
Recombinant Zebrafish mcf2lb Protein, N-GST & C-His
- 库存：
999
- 供应商：
abinScience
- 规格：
50ug
| Product name | Recombinant Zebrafish mcf2lb Protein, N-GST & C-His |
|---|---|
| Catalog No. | ZA481012 |
| Host species | Lyophilized |
| Specificity | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Conjugation | >90% as determined by SDS-PAGE. |
| Species reactivity | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Immunogen | Please contact with the lab for this information. |
| Form | E. coli |
| Storage buffer | XP_009303052.1 |
| Purity | Leu42-Gln193 |
| Clonality | Recombinant |
| Isotype | 45.31 kDa |
| Applications | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Target | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Purification | guanine nucleotide exchange factor DBS isoform X6, mcf2lb |
| Accession | Danio rerio (Zebrafish) |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验Thrombin Cleavage of GST-Fusion protein
activity keeps the enzyme in aliquots at -80°C. Thrombin (MW 37kDa) ~4500units/mg. One unit cleaves theoretical 100ug protein in 16hr at 22°C in buffer. Another possibility is to use high purity enzyme from Sigma # T-6884: 3-10 U of thrombin can be used
Small Scale GST-Tag Purification Under Nature Conditions
Small Scale GST-Tag Purification Under Nature Conditions According to AMERSHAM-BIOSCIENCES-GST fusion protein Handbook (pdf) Aliquot of Cell Pellet after Induction The idea is to aliquot cells after induction
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
expressing each construct (GST alone, GST fusion proteins) into individual 5-ml aliquots of LB broth containing appropriate antibiotic selection. Grow overnight at 37°C with shaking. Although variations of GST fusion protein expression vectors
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