- ￥1268
- abinScience
- France
- ZA451012
- 2026年03月03日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期：
1 year
- 英文名：
Recombinant Zebrafish IL4 Protein, N-GST & C-His
- 库存：
999
- 供应商：
abinScience
- 规格：
50ug
| Product name | Recombinant Zebrafish IL4 Protein, N-GST & C-His |
|---|---|
| Catalog No. | ZA451012 |
| Host species | Lyophilized |
| Specificity | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Conjugation | >90% as determined by SDS-PAGE. |
| Species reactivity | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Immunogen | Please contact with the lab for this information. |
| Form | E. coli |
| Storage buffer | NP_001164211.1 |
| Purity | Val14-Thr127 |
| Clonality | Recombinant |
| Isotype | 40.85 kDa |
| Applications | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Target | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Purification | interleukin-4 precursor, il4 |
| Accession | Danio rerio (Zebrafish) |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验-His-Pro-Gln-Phe-Glu-Lys) 可放在N- 或 C-terminus 不影响蛋白折叠和功能，无需移除 分子量 1058Da Twin-Strep-tag® （也有叫做双Strep-tag®II） 28 aa 标签(WSHPQFEK-GGGSGGGSGG-SA-WSHPQFEK) 两段Strep-tag®II标签序列 较Strep-tag®II对于Strep-Tactin®有更高亲和力 分子量 2887Da 二者均为小标签，不会对蛋白质的结构和功能活性产生影响，因此通常不需要在纯化
Thrombin Cleavage of GST-Fusion protein
activity keeps the enzyme in aliquots at -80°C. Thrombin (MW 37kDa) ~4500units/mg. One unit cleaves theoretical 100ug protein in 16hr at 22°C in buffer. Another possibility is to use high purity enzyme from Sigma # T-6884: 3-10 U of thrombin can be used
科研人必看：多肽、蛋白质、重组蛋白分不清？ 应用场景有哪些？定制如何选？
动物细胞表达：最接近天然状态的表达平台，提供完整的人源化翻译后修饰 (如糖基化、磷酸化)，适合重组抗体、细胞因子等需要复杂修饰的蛋白。 昆虫细胞表达：基因承载容量大，支持大分子量蛋白和激酶的表达。 『多种融合标签』 标签的选择直接影响纯化效率和下游应用，MCE 支持多种融合标签，可根据实验需求灵活搭配。 常规纯化标签：His 标签 (镍柱纯化，分子量小)、GST 标签 (Glutathione 亲和纯化，兼具促溶作用)、Fc 标签 (Protein A/G 亲和纯化，适合免疫相关实验)、Flag 标签
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