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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- 保质期:
1 year
- 英文名:
Recombinant Zebrafish bad Protein, N-His-SUMO
- 库存:
999
- 供应商:
abinScience
- 规格:
50ug

| Product name | Recombinant Zebrafish bad Protein, N-His-SUMO |
|---|---|
| Catalog No. | ZA435012 |
| Host species | Lyophilized |
| Specificity | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Conjugation | >90% as determined by SDS-PAGE. |
| Species reactivity | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Immunogen | Please contact with the lab for this information. |
| Form | E. coli |
| Storage buffer | NP_001257524.1 |
| Purity | Ser56-Gly109 |
| Clonality | Recombinant |
| Isotype | 18.39 kDa |
| Applications | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Target | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Purification | BCL2 associated agonist of cell death b isoform 1, badb |
| Accession | Danio rerio (Zebrafish) |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验)有在5℃下进行切割的蛋白酶,Qiagen也有4℃下进行切割的蛋白酶,这样可以最大程度地防止蛋白降解。同时Qiagen还通过基因改造让蛋白酶也带上标签,这样一步就可以同时把蛋白酶和外源蛋白上含有的标签一并切除。现在为你介绍NEB是怎么解决这个问题的吧。利用内含肽,出除标签不留痕NEB是最早开发内含肽(intein)技术的公司。intein称为“内含肽”,在还原条件下、或者pH值或温度条件改变下会发生N端自剪接,兼有蛋白酶和蛋白连接酶的特性,与内含子在RNA水平自剪接的作用方式相似。IMPACT表达的目的蛋白
信息 1、Recombinant Gene Expression : Reviews and Protocols Editor(s): Paulina Balbás1, Argelia Lorence2 Affiliation(s): (1),Centro de Investigación en Biotecnología,CuernavacaMéxico (2)Virginia Polytechnic Institute,State University,BlacksburgVA
前言: NusA融合蛋白表达系统于9年前(1999年)由Davis发明,并在2000年由Novagen首先进行商业化设计和开发,特别推荐用于以NusA作为N端标签时提高在大肠杆菌中难溶的重组表达蛋白的可溶性。NusA融合蛋白表达系统面市至今,已经通过一些高通量表达筛选的评估确认其在可溶性改善方面的有效性。关于NusA融合表达蛋白去除或不去除NusA标签,目的蛋白仍然具有活性的报道也有很多。正是由于NusA标签系统的这种特性,使其成为目前在大肠杆菌表达体系中使用最为广泛的三个融合标签
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