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无锡莱弗思生物实验器材有限公司
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文献和实验Enhancement of Ribozyme Function by RNA Binding Proteins
The hammerhead ribozyme motif can be engineered to function as a sequence-specific endoribonuclease for use as a specific inhibitor of gene expression (1 ,2 ). However, efficient inhibition often requires high ribozyme concentrations
Ribonuclease inhibitor (RI) is a cytoplasmic protein of ∼50 kDa that tightly binds and inhibits ribonucleases (RNases) from the pancreatic superfamily (1 ). Diverse RNases with very limited sequence similarities, including RNase
DNA-Directed Assembly Microarray for Protein and Small Molecule Inhibitor Screening
. Herein, we describe the detailed experimental protocols in performing the DNA-directed assembly platform for antibody microarray, a RNA polymerase-DNA binding microarray, and a drug-screening microarray.
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