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文献和实验PEAS Labeling Gcn4-cys James Fishburn, Hahn Lab March 2006 Notes 10 mCi 125I = 4.6 nmol (see spec. sheet for calculation) 4.6 nmol Gcn4(1-118,209-281) = 103 microgram (M.W. 22,400) PEAS has low solubility in aqueous
Difference Gel Electrophoresis Based on Lys/Cys Tagging
Before separation, proteins of different biological samples are labeled with different fluorescent dyes, the CyDye™ DIGE Fluors. Currently three dyes with spectrally different excitation and emission wavelengths are available. This allows
Engineering Cys2His2 Zinc Finger Domains Using a Bacterial Cell-Based Two-Hybrid Selection System
Synthetic Cys2His2 zinc finger domains with novel DNA-binding specificities can be identified from large randomized libraries using selection methodologies such as phage display. It has been previously demonstrated that a bacterial cell-based
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