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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Immunotag™ Rat Pkm2 (Pyruvate kinase isozymes M1/M2) ELISA
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
96wellplate
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文献和实验at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
, and proven effective. Finally, it is necessary to correlate any induced phenotypic change with the extent of knockdown induced by the siRNA. Ambion's Silencer™ Pre-designed siRNAs for >34,000 human, mouse, and rat genes in the NCBI RefSeq database eliminate
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