Hotstart dNTP Mix (25 mM each)

Hotstart dNTP Mix is an innovative product in the field of PCR technology. It is a specially chemically modified deoxyribonucleotide that cannot participate in phosphodiester bond formation at room temperature. After heat activation, it converts to regular dNTPs, enabling participation in PCR amplification. This product significantly reduces non-specific amplification caused by non-specific annealing or primer dimers.

Description

Hotstart dNTP Mix is an innovative product in the field of PCR technology. It is a specially chemically modified deoxyribonucleotide that cannot participate in phosphodiester bond formation at room temperature. After heat activation, it converts to regular dNTPs, enabling participation in PCR amplification. This product significantly reduces non-specific amplification caused by non-specific annealing or primer dimers.

Hotstart dNTP Mix can be stably stored at -20°C for at least a year. It is compatible with various primers and thermostable DNA polymerases, making it suitable for standard PCR, fast thermal cycling PCR, multiplex PCR, and real-time quantitative PCR. Its broad applicability and cost-effectiveness make it an ideal choice for optimizing PCR technology.

Features

• Effective Sealing at Room Temperature: To demonstrate the sealing effect of Hotstart dNTP Mix, we conducted a verification test within an isothermal PCR (LAMP) system, where the temperature throughout the PCR process does not exceed 60°C. After placing the complete premixed system for 3 days, the results showed that, compared to regular dNTPs, Hotstart dNTP Mix exhibited complete sealing with no signal detected.
• High Release Efficiency After Heat Activation: Under variable temperature PCR conditions, Hotstart dNTP Mix does not require separate heating and can be fully released during the normal PCR procedure. Despite its rapid release, the amplification efficiency of Hotstart dNTP Mix remains consistent with that of regular dNTPs. Under isothermal LAMP conditions, the sealing effect of Hotstart dNTP Mix can be quickly removed by heating the components individually for 30 seconds, with amplification efficiency nearly identical to that of regular dNTPs.
• Effective Reduction of Non-Specific Amplification: To investigate the effectiveness of Hotstart dNTP Mix in reducing non-specific amplification, we conducted capillary electrophoresis experiments. In a highly multiplexed STR system, we compared the performance of Hotstart dNTP Mix with that of regular dNTP products. The results showed that hot-start DNA polymerase combined with regular dNTPs produced a significant amount of non-specific amplification. In contrast, the combination of Hotstart dNTP Mix with hot-start DNA polymerase significantly reduced non-specific amplification.