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- 英文名：
Immunotag™ Bovine Nucleolar and spindle-associated protein 1 ELISA Kit
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
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- 规格：
96wellplate
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文献和实验of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine 2000™ or stock 3.4µM
should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
Production of Monoclonal Antibody by DNA Immunization with Electroporation
associated with problematic antigens: low abundance and protein instability and use of recombinant proteins that lack posttranslational modifications. This chapter describes the use of a DNA-based immunization protocol for the production of mAb
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