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- 详细信息
- 技术资料
- 英文名：
Mouse Absolute Mitochondrial DNA Copy Number Quantification qPCR Assay Kit
RayBio®线粒体DNA拷贝数绝对定量试剂盒（H-mtDNA-CNQ）是一种基于TaqmanTM探针的qPCR检测方法，用于人类mtDNA-CN的特定定量测量。 mtDNA引物和探针均特异性靶向人类mtDNA上最保守的区域之一，不会扩增出核基因组DNA上的任何脱靶序列。同时，新增单拷贝基因（IFNB1-人类 /36B4-小鼠）的引物和探针特异性识别和扩增110 bp长的核基因组DNA，作为数据归一化的参考。优化后的PCR配方在较宽的线性动态范围内提供了优越的特异性和效率。 本试剂盒可在一次反应中通过线粒体（mt）和核(n) DNA的比较来测定人类线粒体DNA拷贝数。
Kit Components
| Component | Size / Description |
|---|---|
| 2X Probe qPCR Master Mix | 1 mL / 1 tube |
| Primer and Probe Mix | 0.5 mL / 1 tube |
| Reference DNA (2500 copies/cell) | 20 µL / 1 tube |
| Nuclease-free H2O | 1 mL / 1 tube |
Additional Materials Required
- Fluorescence qPCR instrument capable of reading FAM (494nm maximum absorption, 518nm maximum emission) and VIC (532nm maximum absorption, 552nm maximum excitation) channels
- Sterile nuclease-free pipette tips (barrier tips recommended)
- Microfuge tubes
- Compatible PCR Plate
- Microcentrifuge
- Vortex Mixer
Assay Procedure Summary
- Prepare all reagents, samples and standards as instructed.
- Load the PCR reaction mix and samples.
- Seal the PCR reaction tubes or plate.
- Set up the PCR Amplification.
- Export the results.
- Calculate mtDNA copy number.
Specificity
Non-mouse DNA and no DNA samples have higher Cq values than mouse DNA, which means there is high specificity for mtDNA and 36B4 amplification.
Reproducibility
CV < 5% for Cq value and CV < 20% for CopiesCell when the input DNA ≥ 0.3 ngreaction.
Typical Data
Storage/Stability
The kit can be stored at -20°C for a period of 12 months prior to opening. After opening, the reagents are valid for at least 6 months if stored at -20°C. Avoid repeated freeze-thaw cycles.
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小鼠线粒体DNA qPCR试剂盒
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