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- 2025年10月07日
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- 技术资料
- 保存条件：
负80摄氏度
- 保质期：
3个月
- 英文名：
Ar A4 Agrobacterium Strain
- 库存：
50
- 供应商：
LSM BIO
- 规格：
100ul
Ar A4 Agrobacterium Strain
STR3109 100ul
Store at -80鈩?/span>
Ar A4 Agrobacterium Strain Description
Resistance: Kan
Culture medium: LB
Strain category: Agrobacterium tumefaciens
Culture conditions: 30 centigrade aerobic, LB
Transformation of plasmids: electronic or chemicallly transformation
Preservation method: 30% glycerin, -80鈩?br /> Type Agrobacterium tumefaciens
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. Please kindly culture it as soon as possible then harvest and store at -80鈩?
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文献和实验Article: Construction of a genome-editing system for the thermophilic actinomycete streptomyces thermodiastaticus K5 strain Authors: Kenji Yamagishi 1, Masakazu Ike 1, Ken Tokuyasu 1 Biosci Biotechnol Biochem . 2024 Nov 12:zbae157. PMID: 39533823 DOI: 10.1093/bbb/zbae157
Agrobacterium growth and transformation
. This is not very efficient (~200 colonies per microgram of DNA), but it is really easy.1. Pick a single colony of the Agrobacterium strain of choice and inoculate 3 ml of LB (or 2YT) in a 15 ml snap-cap tube (Falcon tube). Grow @ 30℃ overnight on a roller drum. Be sure
Growth and Storage of Agrobacterium
Proper preservation of strains is of great importance to all microbiologists. Working with contaminated or genetically changed cultures, or facing the loss of a strain are annoyances that often may be easily prevented. A reliable preservation
Electroporation of Agrobacterium tumefaciens
Agrobacterium tumefaciens is a soil bacterium that causes tumors on dicotyledonous plants. Virulent strains harbor a large plasmid, the Ti (tumor-inducing) plasmid, which is involved in tumorigenesis. A small segment of this plasmid, the T
技术资料