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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80
- 保质期：
详询
- 英文名：
【干冰】HSVd TaqMan RT-PCR Kit
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
100Reactions
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文献和实验using the AllPrep DNA/RNA 96 Kit. Real-time RT-PCR analysis of the PGK1 transcript was performed using a primer-probe set that could amplify and detect both mRNA and genomic DNA sequences. The flat amplification plot for the no RT control (-RT) indicates
TaqMan Primer and Probe Design
Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit Instruction Design of Probes Keep the G-C content in the 20 to 80% range. Avoid runs of an identical nucleotide. This is especially true for guanine, where runs
The RT-PCR kit contains polymerase and all necessary reagents to perform single tube RT and PCR. However, we will break the reaction into two discrete steps, RT (reverse transcription to convert mRNA to cDNA) and PCR
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