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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80
- 保质期:
详询
- 英文名:
【干冰】HLVd/LCV/CanCV TaqMan RT-PCR Kit
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
100Reactions
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文献和实验A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
TaqMan Primer and Probe Design
Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit Instruction Design of Probes Keep the G-C content in the 20 to 80% range. Avoid runs of an identical nucleotide. This is especially true for guanine, where runs
The RT-PCR kit contains polymerase and all necessary reagents to perform single tube RT and PCR. However, we will break the reaction into two discrete steps, RT (reverse transcription to convert mRNA to cDNA) and PCR
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