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- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
详询
- 英文名:
FBS Exosome Depletion Kit I (Slurry Format)
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
6Preps
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文献和实验A c-Kit (2B8) FITC-IgG2b, 01904D FITC-IgG2b Isotype control, 11184C Fc RII block (clone 2.4G2), 01241D Media: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated
(Invitrogen) at a 1:3 ratio (e.g. 1µl plasmid to 3µl lipofectamine). The plasmid and lipofectamine are mixed together, vortexed, pulse spun, and then 50µl of DMEM (without Fetal bovine serum, FBS) added. The entire mixture is again vortexed followed by a pulse
Affinity chromatography (with HIS-tagged proteins)
. Store both solutions on ice. 4) Prepare the nickel resin. 50uL (settled volume) of resin is required for each experimental and control column. Transfer the appropriate volume of slurry into two siliconized eppendorf tubes, one for the control
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