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铵、0.2 g 柠檬酸钠和 64 ml 水，灭菌后冷却至 50°C。加入 64 ml 培养基到这个瓶中，4 ml 10 mg/ml 尿嘧啶、1.6 ml 50% 葡萄糖、0.32 ml 1 mol/L 硫酸镁、0.66 ml 30 mg/ml Km 和 20 μl 100 mg/ml 硫胺。 ( 8 ) 酵母抽提物-蛋白胨-葡萄糖（YPD)：20 g 蛋白胨、10 g 酵母抽提物和 20 g 葡萄糖溶于 1 L 水中。用平板时，加入 20 g/L 菌用琼脂糖。 ( 9 ) 酵母全价（YC

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): N ‐Benzoyl‐2′‐deoxyadenosine phosphoramidite N ‐Benzoyl‐2′‐deoxycytidine phosphoramidite or N ‐Acetyl‐2′‐deoxycytidine phosphoramidite