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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
JOE azide, 5-isomer, 100 mg
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
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- 规格：
100mg
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文献和实验Replication timing by density transfer
. (I add 0.1 mg/ml ampicillin if I'm feeling paranoid.) The doubling time of RM14-3a is ~ 2.6 hr at 23o C in glucose and 6-8 hr (depending on aeration) in acetate--longer if you're selecting for a plasmid. RM14-3a is MATa, cdc7-1ts , bar1, trp1-289
Purification of Antiserum or Ascites by Protein A/G Chromatography
A and protein G bind about 20 mg of IgG per ml of conjugated agarose. Therefore, the binding capacity of a 10 ml (5 ml) column is 200 mg (100 mg) of IgG. A high-titer rabbit antiserum has roughly 5 mg/ml of IgG, mouse ascites has roughly 10 mg/ml of IgG
DETECTION OF RECOMBINANT PROTEINS BY ELISA
2CO 3 (0.012M) 0.02 g MgCl 2 (100 µl 1 M stock; 1 mM) 200 µl 10% sodium azide Add Na 2CO 3 slowly while stirring. Adjust pH to 8.6 and QS to 100 ml with H 2O. Substrate Solution (1 mg/ml
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