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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
FAM CPG 500, 6-isomer, 1 g
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
1g
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文献和实验CpG Island length variation-DNA Methylation, Histone and
----------------------- Hi Chris, Based on Gardiner-Garden's criteria, CpG islands are generally 500 bp in length [1]. Later, Jones et al [2] modified the criteria and suggested that regions of DNA of greater than 500 bp with a G+C equal to or greater than 55% and observed
Methylated CpG Island Amplification
hybridization solution (e.g. BLOTTO) Random-primed DNA labeling kit Wash solutions (Wash1 2xSSC, 0.1%SDS; Wash2 0.1XSSC, 0.1%SDS) 1.2. RDA and Cloning PCR Products 3 X EE buffer : 30 mM EPPS (SIGMA) pH 8.0, 3 mM
Methylated CpG Island Amplification (MCA)
Methylated CpG island Amplification Protocol written by Minoru Toyota 2. Materials 2.1. MCA Restriction enzymes SmaI, XmaI T4 DNA ligase Taq DNA polymerase 10X PCR reaction buffer: 670mM Tris-HCl, pH
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