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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
BDP® FL alkyne, 50 mg
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
50mg
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文献和实验实验步骤 1. Measure out the amount of Taq DNA polymerase needed. When using a reaction cocktail, determine the total number of units in the mix. 2. Add an amount of “equivalent units” of Platinum® Taq
Extraction of DNA using DNAzol® Reagent
. Use a loosely fitting homogenizer, with a tolerance of 0.1-0.15 mm or higher. Homogenize 25-50 mg tissue in 1 ml of DNAzol® Reagent by applying as few strokes as possible. Typically, 5-10 strokes are required for complete homogenization. Small amounts
使用 Platinum® Taq DNA 聚合酶 (Platinum® Taq DNA Polymerase),对靶序列进行定性验证
1. Add the following components to a sterile 0.5-ml microcentrifuge tube: 10X PCR Buffer, Minus Mg 5 µl 10 mM dNTP mixture 1 µl 50 mM MgCl2 1.5 µl Primer mix (10 µM
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