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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
AF 568 carboxylic acid, 25 mg
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
25mg
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文献和实验Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
50% glycerol100 mM Tris-HCl pH8 @ 25 degrees 100 microliters 1 M Tris pH8300 mM KCl 300 microliters 1 M KCl25 mM MgCl2 25 microliters 1 M MgCl2500 micrograms/ml BSA 5 microliters 100 mg/ml BSA170 microliters H2Ooptional: add 25 microliters saturated
Acid Phosphatase for Glycol Methacrylate Sections Procedure
, sodium salt (Sigma) - 40 mg N.N-dimethylformamide - 4 ml Pararosaniline Solution Pararosaniline (C.I.# 42500) - 2 g 2N HCl - 50 ml Use heat to dissolve, filter when cool and store at 4°C. Sodium Nitrite Solution
Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
% ammonium persulfate 30 microliters TEMED pH 8.3 with acetic acid at room temp. Adjust volume to 500 ml. Running buffer is 1X TGOE buffer [Note that there is no Mg or EDTA in both the gel and in the running buffer.]
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