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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Alpha-Dystroglycan, Bovine, mAb 2238, 100 ug
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
100UG
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文献和实验Dual- and Triple-Color Fluorospot
, in PBS containing 0.1% bovine serum albumin (BSA) (seeNote 8). Add 100 μl/well and leave the plate for 2 h at room temperature. c. Wash the plate as in step 6 above. d. Dilute secondary detection reagents, i.e., mAb anti-FITC
800/1200 L0007 Mouse anti-Human CD3 mAb, Biotin 100ug/500ug 800/2500 L0008 Mouse anti-Human CD4 mAb,FITC 50T/100T 800/1200 L
Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
and volumes for ppt.need 1.4ml ppt/100mm (half is 2x HeBS, half is DNA/CaCl2 mixture).20-30ug of total DNA required per 100mm plate.5-20ug reporter plasmid.3-4ug alpha-globin internal control.pUC19 to bring to 20-30ug total DNA.Aliquot volume of 2x HeBS
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