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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
BPDE-DNA, mAb 5D11, 100 ug
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
100UG
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文献和实验or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
concentration) 6. resuspend DNA in 20 ul TE 7. treat with 40 ug/ml RNAse for 1hr at 37 degrees C 8. run half of each sample on a 1.5% agarose gel with EtBr with an appropriate DNA sizing ladder (sheared DNA should run between 100 bp and 1000 bp)
to 100 ml FE (formamide/EDTA): 5:1 (v/v) formamide:50 mM EDTA 10 μl ddH2 O 10 μl 100 mM EDTA 100 μl deionized formamide make fresh 10X Fill-in/Kinase buffer : (500 mM Tris-HCl, pH 7.6, 100 mM MgCl2, 10 mM DTT, and 50 ug/ml BSA in double
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