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T25
RK-13细胞RK-13细胞RK-13兔肾细胞
Cell line name RK 13
Synonyms RK-13; RK13; Rk13; R.K.13; GL RK/13; GLRK 13; GLRK/13; GLRK13; Glaxo Laboratories Rabbit Kidney 13
Accession CVCL_3155
Resource Identification Initiative To cite this cell line use: RK 13 (RRID:CVCL_3155)
Comments Virology: Susceptible to infection by herpes simplex virus, ps vaccinia virus, rabbitpox virus, myxoma virus, simian adenoviruses, and rubella virus (ATCC=CCL-37).
Virology: Susceptible to infection by SARS coronavirus (SARS-CoV) (PubMed=16494729).
Virology: Persistently infected by bovine viral diarrhea virus (BVDV) (PubMed=7989438).
Doubling time: ~75 hours (PubMed=4297026).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Cell type: Epithelial cell of kidney; CL=CL_0002518.
Species of origin Oryctolagus cuniculus (Rabbit) (NCBI Taxonomy: 9986)
Hierarchy Children:
CVCL_0A22 (elkRK13) CVCL_4477 (RK 13/1) CVCL_M730 (RK 13/91)
CVCL_JK51 (RK-13-KY) CVCL_A0TL (RK-13/CD40L) CVCL_0I47 (RK13-DrdI)
CVCL_B0BE (RK13-UL11) CVCL_B0BD (RK13-UL37) CVCL_B0BC (RK13-UL51)
CVCL_W712 (RK13.6) CVCL_U156 (Rov9)
Sex of cell Sex unspecified
Age at sampling 5W
Category Spontaneously immortalized cell line
PubMed=13881104; DOI=10.1016/0014-4827(61)90464-5
Coombs R.R.A., Gurner B.W., Beale A.J., Christofinis G.J., Page Z.
The identity of three strains of cells derived from pig or rabbit kidney tissue, checked by means of the mixed agglutination reaction.
Exp. Cell Res. 24:604-605(1961)
PubMed=13881105
Coombs R.R.A.
Identification and characterization of cells by immunologic analysis, with special reference to mixed agglutination.
Natl. Cancer Inst. Monogr. 7:91-103(1962)
DOI=10.1016/S0140-6736(63)90434-3
Beale A.J., Christofinis G.J., Furminger I.G.S.
Rabbit cells susceptible to rubella virus.
Lancet 282:640-641(1963)
PubMed=4297026; DOI=10.1002/path.1700950204
Christofinis G.J., Beale A.J.
Some biological characters of cell lines derived from normal rabbit kidney.
J. Pathol. Bacteriol. 95:377-381(1968)
PubMed=6988327; DOI=10.1007/BF02831503
O'Brien S.J., Shannon J.E., Gail M.H.
A molecular approach to the identification and individualization of human and animal cells in culture: isozyme and allozyme genetic signatures.
In Vitro 16:119-135(1980)
PubMed=7989438; DOI=10.1016/0166-0934(94)90120-1
Bolin S.R., Ridpath J.F., Black J.W., Macy M.L., Roblin R.O. 3rd
Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus.
J. Virol. Methods 48:211-221(1994)
PubMed=16494729; DOI=10.3201/eid1201.050496; PMCID=PMC3291385
Kaye M., Druce J., Tran T., Kostecki R., Chibo D., Morris J., Catton M., Birch C.
SARS-associated coronavirus replication in cell lines.
Emerg. Infect. Dis. 12:128-133(2006)
PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)
PubMed=25565633; DOI=10.1371/journal.pone.0117154; PMCID=PMC4286239
Oelschlegel A.M., Geissen M., Lenk M., Riebe R., Angermann M., Schaetzl H., Groschup M.H.
A bovine cell line that can be infected by natural sheep scrapie prions.
PLoS ONE 10:E0117154-E0117154(2015)
PubMed=34737324; DOI=10.1038/s41598-021-00779-5; PMCID=PMC8569163
Lung O., Candlish R.C., Nebroski M., Kruckiewicz P., Buchanan C., Moniwa M.
High-throughput sequencing for species authentication and contamination detection of 63 cell lines.
Sci. Rep. 11:21657-21657(2021)
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文献和实验*发表【中文论文】请标注：由博辉生物科技（广州）有限公司提供； *发表【英文论文】请标注：From Bohui Biological Technology (Guangzhou) Co., Ltd.
Nat Methods：陈玲玲/杨力/李劲松等开发新技术，实现环状 RNA 功能性筛选和研究
Methods 官网）前述实验结果表明 RfxCas13d 是一种高效、特异的 circRNA 敲除工具。研究团队构建了靶向 9 个人类细胞系中 2908 个高表达 circRNA 的 BSJ-gRNAs 文库，尝试使用该工具鉴定潜在的参与细胞增殖的候选 circRNA。 BSJ-gRNA 文库构建（图源：Nature Methods 官网） 研究团队将 BSJ-gRNA-RfxCas13d-HT29 细胞转移到裸鼠体内进行体内功能缺失（loss-of-function，LOF）表型筛筛选，注射 22 天后
Crystallization of Kinesin Family Motor Proteins
.67 Åc=74.13 Å1.9 ÅMolecular replacement rK379PDB: 3KINP212121a=72.2 Åb=91.9 Åc=141.7 Å3.0 ÅMultiple isomorphous replacement Kar3 382-729PDB: 3KARP21a=44.1 Åb=81.2 Åc=48.3 Åß=105.8°2.3 ÅMolecular replacement and phases of three heavy atom derivatives
属节肢介体病毒中的披盖病毒（Togavirus ）群，为风疹的病原病毒。由 T.H.Weller 与 F.A.Neva （ 1962 ）及 P.D.Parkman 等（ 1962 ）自风疹患者的咽部洗涤液中分离到的。病毒粒子具多形性， 50 — 85 纳米，有包被。粒子中含有分子量为 2.6 — 4.0 × 10 6 的 RNA （感染性核酸）。乙醚和 0.1 ％的脱氧胆酸盐可使其钝化，在热中亦弱化。在兔或猪等动物的肾细胞中、或 BSC-1 、 RK-13 、 BHK-21 等细胞
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