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- 详细信息
- 技术资料
- 年限:
5 weeks
- 细胞形态:
上皮样
- 库存:
大量
- 器官来源:
肾脏
- 生长状态:
贴壁生长
- 物种来源:
其他
- 是否是肿瘤细胞:
0
- 相关疾病:
正常
- 运输方式:
冻存运输
- ATCC Number:
CCL-37™
| Designations: | RK13 | ||
| Depositors: | BC Meyer | ||
| Biosafety Level: | 2 [Cells contain Bovine Viral Diarrhea Virus (BVDV) ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Oryctolagus cuniculus | ||
| Morphology: | epithelial |
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| Source: | Organ: kidney Disease: normal |
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| Cellular Products: | keratin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Age: | 5 weeks | ||
| Comments: | The cells are positive for keratin by immunoperoxidase staining.The cells are positive for bovine viral diarrhea virus (BVDV). | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended Medium Renewal: Twice per week |
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| Preservation: | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101 Cell culture tested DMSO:ATCC 4-X |
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| References: | 18385: Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708 22145: . . Lancet 2: 640, 1963. 22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438 26192: . . Lancet 2: 593, 1963. 28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321 |
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RK13
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