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T25
Marc-145/Marc-145细胞系/Marc-145细胞株/Marc-145猴胚胎肾上皮细胞
Cell line name MARC-145
Synonyms Marc-145; MARC 145; Marc 145; MARC145; Marc145; Meat Animal Research Center-145
Accession CVCL_4540
Resource Identification Initiative To cite this cell line use: MARC-145 (RRID:CVCL_4540)
Comments Group: Non-human primate cell line.
Group: Patented cell line.
Problematic cell line: Misidentified. Parent cell line (MA-104) has been shown to be from African Green monkey instead of Rhesus macaque.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00621.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-12219.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-12230.
Virology: Highly susceptible to infection by porcine reproductive and respiratory syndrome virus (PRRSV).
Omics: Transcriptome analysis by microarray.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin Chlorocebus pygerythrus (Vervet monkey) (Cercopithecus pygerythrus) (NCBI Taxonomy: 60710)
Hierarchy Parent: CVCL_3845 (MA-104)
Children:
CVCL_1T05 (LN 14832) CVCL_B6C8 (MARC-145 PBD-1+EGFP clone 9/D6) CVCL_VG41 (MARC-145 pig CD163)
CVCL_HA70 (MARC-Nsp11)
Sex of cell Sex unspecified
Age at sampling Fetus
Category Spontaneously immortalized cell line
Publications
PubMed=8257302; DOI=10.1007/BF01313785
Kim H.S., Kwang J., Yoon I.J., Joo H.S., Frey M.L.
Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line.
Arch. Virol. 133:477-483(1993)
Patent=US5690940
Joo H.S.
Low pathogencity PRRS live virus vaccines and methods of preparation thereof.
Patent number US5690940, 25-Nov-1997
PubMed=17081295; DOI=10.1186/1743-422X-3-90; PMCID=PMC1635561
Cafruny W.A., Duman R.G., Wong G.H.W., Said S., Ward-Demo P., Rowland R.R.R., Nelson E.A.
Porcine reproductive and respiratory syndrome virus (PRRSV) infection spreads by cell-to-cell transfer in cultured MARC-145 cells, is dependent on an intact cytoskeleton, and is suppressed by drug-targeting of cell permissiveness to virus infection.
Virol. J. 3:90.1-90.17(2006)
PubMed=23148668; DOI=10.1186/1743-422X-9-267; PMCID=PMC3546013
Provost C., Jia J.-J., Music N., Levesque C., Lebel M.-E., del Castillo J.R.E., Jacques M., Gagnon C.A.
Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line.
Virol. J. 9:267.1-267.14(2012)
PubMed=28046048; DOI=10.1371/journal.pone.0169391; PMCID=PMC5207746
Dotti S., Lombardo T., Villa R., Cacciamali A., Zanotti C., Andreani N.A., Cinotti S., Ferrari M.
Transformation and tumorigenicity testing of simian cell lines and evaluation of poliovirus replication.
PLoS ONE 12:E0169391-E0169391(2017)
PubMed=29682543; DOI=10.1155/2018/4315861; PMCID=PMC5841097
Wu X.-J., Qi J., Cong X.-Y., Chen L., Hu Y., Yoo D.-W., Wang G.-S., Tian F.-L., Li F., Sun W.-B., Chen Z., Guo L.-H., Wu J.-Q., Li J., Wang J.-B., Zhao X.-M., Du Y.-J.
Establishment and characterization of a high and stable porcine CD163-expressing MARC-145 cell line.
BioMed Res. Int. 2018:4315861.1-4315861.9(2018)
PubMed=33268540; DOI=10.1128/jcm.01757-20; PMCID=PMC8106717
Yim-im W., Huang H.-Y., Park J., Wang C., Calzada-Nova G., Gauger P., Harmon K., Main R., Zhang J.-Q.
Comparison of ZMAC and MARC-145 cell lines for improving porcine reproductive and respiratory syndrome virus isolation from clinical samples.
J. Clin. Microbiol. 59:e01757.20.1-e01757.20.15(2021)
PubMed=34205087; DOI=10.3390/vaccines9060594; PMCID=PMC8229515
Xie J.-X., Vereecke N., Theuns S., Oh D.-Y., Vanderheijden N., Trus I., Sauer J., Vyt P., Bonckaert C., Lalonde C., Provost C., Gagnon C.A., Nauwynck H.J.
Comparison of primary virus isolation in pulmonary alveolar macrophages and four different continuous cell lines for type 1 and type 2 porcine reproductive and respiratory syndrome virus.
Vaccines (Basel) 9:594.1-594.13(2021)
PubMed=34737324; DOI=10.1038/s41598-021-00779-5; PMCID=PMC8569163
Lung O., Candlish R.C., Nebroski M., Kruckiewicz P., Buchanan C., Moniwa M.
High-throughput sequencing for species authentication and contamination detection of 63 cell lines.
Sci. Rep. 11:21657-21657(2021)
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文献和实验*发表【中文论文】请标注：由博辉生物科技（广州）有限公司提供； *发表【英文论文】请标注：From Bohui Biological Technology (Guangzhou) Co., Ltd.
Marc 145细胞培养和维持有关资料综述一、 细胞及培养1、Marc145细胞上皮样细胞，来源于猴肾细胞，从母细胞（MA 104细胞）克隆而得到，可连续培养。2、生长培养基DMEM（高糖型，含4500mg/L D－葡萄糖、L-谷氨酰胺，和110mg/L丙酮酸钠，不含碳酸氢钠）＋5－10％新生牛血清+ 1%双抗（青霉素和链霉素）的培养液。3、冻存液生长培养基＋10% DMSO4、细胞健康生长的几项注意事项（1）细胞没有支原体等外源因子的感染。（2）培养液的pH值可在7.0－7.3，以7.2为佳
PRRSV(Porcine Reproductive and Respiratory Syndrome Virus,猪繁殖与呼吸综合征病毒) was propagated in MARC-145 cells and purified by sucrose density gradient separation.Polyethylene glycol 8000(Sigma Chemical Co.,St.Louis,MO) at a final concentration of 8%(wt
。母源抗体半衰期为12～14天，一般仔猪在出生后4～8周内可检测到病毒，随母猪生产时的滴度及所用试验方法而异。在污染的环境中，血清学检测为阳性的母猪所产仔猪能在3～6周龄时血清转为阳性。应用巨噬细胞分离病毒以及利用两类血清型病毒的IPMA在实验室容易操作，这种试验可用MARC-145细胞系增殖欧洲型病毒和美洲型病毒操作。使用MARC-145细胞系做间接免疫荧光实验（1FA）能顺利进行PRRSV血清学反应。目前，灵敏度高、特异性强商品化的ELISA试剂盒已经上市。同时，国内学者还开发了乳胶凝集试剂盒，可用
技术资料
